LongAmpHot StartTaq2X Master Mix from New England Biolabs

Product Specs
ItemLongAmpHot StartTaq2X Master Mix
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0533S
Quantity100 reactions
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeMaster Mix
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Competition between skin antimicrobial peptides and commensal bacteria in type 2 inflammation enables survival of S. aureusCell ReportsMay 30, 2023Teruaki Nakatsuji, Samantha L. Brinton, Kellen J. Cavagnero, Alan M. O’Neill, Yang Chen, Tatsuya Dokoshi, Anna M. Butcher, Olive C. Osuoji, Faiza Shafiq, Josh L. Espinoza, et al.(GLL-33),"Genemed Synthesis, Inc.",Custom Peptide LongAmp Hot Start Taq 2X Master Mix,New England BioLabs,Cat#: M0533S Agencourt AMPure XP beads,Beckman Coulter,Cat#: A63881 ,, Critical commercial assays,, ,, PureLink

(2) Capture-seq protocol and TE-reX pipeline guidelines for detection of recombination of repeat elements in short- and long-DNA reads librariesSTAR ProtocolsMarch 1, 2023Giovanni Pascarella, Letizia Straniero, Martin Frith, Piero CarninciIndex Primer Pairs),New England Biolabs,E6440S LongAmp Hot Start Taq 2X Master Mix,New England Biolabs,M0533L High Sensitivity DNA kit,Agilent,5067-4626 RNA 6000 Nano Kit,Agilent,5067-1511 MEGAscript T7 Transcription Kit

(3) A wide foodomics approach coupled with metagenomics elucidates the environmental signature of potatoesiScienceJanuary 20, 2023Anastasia Boutsika, Michail Michailidis, Maria Ganopoulou, Athanasios Dalakouras, Christina Skodra, Aliki Xanthopoulou, George Stamatakis, Martina Samiotaki, Georgia Tanou, Theodoros Moysiadis, et al.Applied Biosystems® QuantStudio® 5 Real-Time PCR System (Thermo Fischer Scientific, Waltham, MA, USA), using a LongAmp Hot Start Taq 2x Master Mix (M0533S, New England Biolabs), and 16S barcoded primers. The 16S Barcoding Kit 1-24 (SQK-16S024, Oxford Nanopore

(4) INSERT-seq enables high-resolution mapping of genomically integrated DNA using Nanopore sequencingGenome BiologyOctober 25, 2022Dimitrije IvanÄiÄ‡, JÃºlia Mir-Pedrol, Jessica Jaraba-Wallace, NÃºria Rafel, Avencia Sanchez-Mejias, Marc GÃ¼ellsingle-tail adapter/tag (STAT)-PCR [29] were performed with LongAmp Hot Start Taq polymerase (NEB, M0533S) using adaptor binding P5_1 and P5_2 primers and corresponding insert primer pairs (see Table S2). For

(5) Targeting DNA polymerase to DNA double-strand breaks reduces DNA deletion size and increases templated insertions generated by CRISPR/Cas9Nucleic Acids ResearchApril 22, 2022Kyung W Yoo, Manish Kumar Yadav, Qianqian Song, Anthony Atala, Baisong Luregion of 4862 bp was amplified by LongAmp® Hot Start Taq 2× Master Mix (New England Biolabs, catalog # M0533) with primers hCLCN5-F3 and hCLCN5-R3. DNA was submitted to the GCB Sequencing and Genomic Technologies

(6) Flow-induced endothelial mitochondrial remodeling mitigates mitochondrial reactive oxygen species production and promotes mitochondrial DNA integrity in a p53-dependent mannerRedox biologyApril 1, 2022Shin J, Hong SG, Choi SY, Rath ME, Saredy J, Jovin DG, Sayoc J, Park HS, Eguchi S, Rizzo V, et al.DNA from each sample were amplified with LongAmp Hot Start Tag 2X Master Mix polymerase Taq (NEB, #M0533S) for long amplicon (LA) and short amplicon (SA). The PCR products were quantified using PicoGreen dye

(7) High-speed rail model reveals the gene tandem amplification mediated by short repeated sequence in eukaryoteScientific ReportsFebruary 10, 2022Haidi Chen, Jingwen Xue, Zhenghou Zhang, Geyu Zhang, Xinyuan Xu, He Li, Ruxue Zhang, Najeeb Ullah, Lvxing Chen, Amanullah, et al.dried and finally resuspended in 100 µl sterile dH2O. Genomic DNA was amplified using a long PCR kit (M0533S, NEB)49. Then the products were collected for sequencing (PacBio Sequel II, CCS model). Gene synteny

(8) Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexonsNature CommunicationsDecember 1, 2021Karim Rahimi, Morten T. VenÃ¸, Daniel M. Dupont, JÃ¸rgen KjemscircRNA. To do RT-PCR for microexon validation, LongAmp Hot Start Taq 2X Master Mix (New England Biolabs, M0533L) was used following the manufacturer’s instruction. Sequence of the primers provided in Supplementary

(9) Generation of mouse conditional knockout alleles in one step using the i-GONAD methodGenome ResearchJanuary 1, 2021Renjie Shang, Haifeng Zhang, Pengpeng Biwith T7 or SP6 primers. Long-range PCR was performed using LongAmp Hot Start Taq 2X Master Mix (NEB M0533S) to examine genomic DNA that was purified by a Monarch Genomic DNA Purification kit (NEB T3010). The

(10) Protocol for Efficient CRISPR/Cas9/AAV-Mediated Homologous Recombination in Mouse Hematopoietic Stem and Progenitor CellsSTAR protocolsJune 19, 2020Tran NT, Trombke J, Rajewsky K, Chu VT.Kit (VioBright™ FITC),Miltenyi Biotec,Cat#130-123-124 LongAmp PCR Master Mix,New England Biolabs,Cat#M0533L KOD Hot Start DNA Polymerase,Millipore,Cat#71086-3 p.JET TOPO cloning Kit,Thermo Fischer,Cat#K1231 T7 endonuclease I

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